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Antibodies bound to the blocking peptide not bind to the epitope within the focus on protein. By comparing the staining with the blocked antibody vs . the antibody by yourself, one can see which staining is certain.

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This guidebook outlines more attainable optimization of mobile and immunoassay parameters to ensure the absolute best success are...

This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best benefits are...

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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

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Blocking peptides are peptides that bind especially on the goal antibody and block antibody binding. These peptide commonly is made up of the epitope regarded via the antibody.

Target Information Cyclin E belongs to your really conserved cyclin family, whose associates are characterized by a remarkable periodicity in protein abundance from the mobile cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins show distinctive expression and degradation styles which lead on the temporal coordination of every mitotic occasion. Cyclin E types a complex with and capabilities being a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates for the G1-S period boundary and it is degraded as cells development as a result of S period.

Overexpression of the gene has long been noticed in several tumors, which leads to chromosome instability, and so could add to tumorigenesis. This protein was identified to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital job in selling mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two supplemental splice variants were described but detailed nucleotide sequence info isn't nevertheless readily available.

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